Jump to menu
DASNR logo

Entomology and Plant Pathology

Dr. Astri Wayadande, Associate Professor, Interim Dir. NIMFFAB

B.S., University of California-Davis, 1983

M.S., University of Missouri, 1987

Ph.D., Ohio State University, 1991


Room/Lab 130D HBRC/210 NRC



National Institute for Microbial Forensics and Food and Agricultural Biosecurity (NIMFFAB)


PLP 5013  Plant Virology Laboratory


1. Feeding physiology and behavior of leafhoppers and other hemiptera.

2. Functional anatomy of leafhoppers with respect to the transmission of plant pathogenic spiroplasmas. 

3. Ecology of leafhopper vectors of grass-infecting viruses and bacteria

4. Anatomy and physiology of salivary gland structures of the hemiptera, especially for the squash bug, Anasa tristis (deGeer).

5. Transmission biology of the cucurbit yellow vine bacterium, Serratia marcescens.

6 Fly transmission of E. coli O157:H7 and Salmonella to pre-harvest leafy greens and fruit.


Wayadande, A., B. Bruton, J. Fletcher, S. Pair, and F. Mitchell. 2005. Retention of the cucurbit yellow vine disease bacterium, Serratia marcescens, through transstadial molt of the vector, Anasa tristis (Hemiptera: Coreidae). Accepted Ann. Entomol. Soc. Am. 00:000-000.

Fletcher, J., U. Melcher, and A. Wayadande. The Phytopathogenic Spiroplasmas. In: The Prokaryotes, M. Dworkin, ed. In Press.

Bruton, B., F. Mitchell, J. Fletcher, S. Pair, A. Wayadande, U. Melcher, J. Brady, B.R. Bextine, and T. Popham. 2004. Serratia marcescens, a phloem-colonizing squash bug transmitted bacterium, is the causal agent of cucurbit yellow vine disease. Plant Dis. 87: 937-944.

Pair, S., B. Bruton, F. Mitchell, J. Fletcher, A. Wayadande, and U. Melcher. 2003. Overwintering squash bugs harbor and transmit the causal agent of cucurbit yellow vine disease. J. Econ. Entomol. 97: 71-78.

Fletcher, J. and A. Wayadande. 2002. Fastidious vascular-colonizing bacteria. Plant Health Instructor. DOI: 10.1094/PHI-I-2002-1218-02.

Bextine, B., A. Wayadande, B. Bruton, S. Pair, F. Mitchell, and J. Fletcher. 2001. Effect of insect exclusion on the incidence of yellow vine disease and of the associated bacterium in squash. Plant Dis. 85:875-878.

Yu, J., A.C. Wayadande, and J. Fletcher. 2000. Spiroplasma citri surface protein P89 implicated in adhesion to cells of the vector, Circulifer tenellus. Phytopathology 90:716-722.

Fletcher, J., A.C. Wayadande, U.K. Melcher, and F. Ye. 1999. The phytopathogenic mollicute-insect interface: A closer look. Phytopathology 88:1351-1358.

Kwon, M.O., A.C. Wayadande, and J. Fletcher. 1999. Spiroplasma citri movement into the intestines and salivary glands of its leafhopper vector, Circulifer tenellus. Phytopathology 89:1144-1151.

Wayadande, A.C. and J. Fletcher. 1998. Development and use of an established cell line of the leafhopper Circulifer tenellus, to characterize Spiroplasma citri vector interactions. J. Invert. Pathol. 72:126-131.

Wayadande, A.C., G.R. Baker, and J. Fletcher. 1997. Comparative ultrastructure of the salivary glands of two phytopathogen vectors, the beet leafhopper, Circulifer tenellus (Baker), and the corn leafhopper, Dalbulus maidis DeLong and Wolcott (Homoptera: Cicadellidae). J. Insect Morphol. and Embryol. 26:113-120.

Document Actions